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Precise H1N1 swine influenza detection using stuffer-free multiplex ligation-dependent probe amplification in conformation-sens

작성자
pmrc
작성일
2018-05-16 21:47
조회
296
Chung, B., Shin, G. W., Hwang, H. S., Chung, Y. J., Jung, S. W., & Jung, G. Y. (2012). Precise H1N1 swine influenza detection using stuffer-free multiplex ligation-dependent probe amplification in conformation-sensitive capillary electrophoresis. Analytical biochemistry, 424(1), 54-56.

IF(2014): 2.219

Abstract

The H1N1 influenza virus has spread worldwide to become pandemic. Here, we developed a new method to discriminate various types of influenza A, including H1N1, using stuffer-free multiplex ligation-dependent probe amplification based on a conformation-sensitive separation method, namely capillary electrophoresis–single-strand conformation polymorphism. Unlike conventional methods, our approach precisely detects five relevant gene markers permitting confirmation of infection.